GMP Human Betacellulin Protein

Betacellulin (BTC)​ is an epithelial growth factor belonging to the epidermal growth factor (EGF) family, primarily known for its potent mitogenic and differentiating effects on pancreatic and other epithelial tissues. In stem cell-based pancreatic differentiation protocols, Betacellulin plays a critical role in the final stages of endocrine maturation. It promotes the functional maturation of pancreatic endocrine precursors, particularly enhancing the differentiation and maturation of pancreatic progenitor cells into glucose-responsive, insulin-secreting beta cells. Additionally, Betacellulin contributes to the expansion and survival of beta-like cells, making it a key component in protocols aimed at generating functional islet organoids or beta-cell clusters for diabetes research and cell therapy development.

• Specific Pancreatic Differentiation Driver: Efficiently drives stem cell differentiation into pancreatic progenitor cells and mature beta islet cells in combination with Activin A.
• Reliable Large-Scale Supply: Ensures batch production capacity exceeding 1 gram, supporting robust and consistent manufacturing scale-up.
• Cost-Effective Solution: Achieves up to 50% cost reduction compared to conventional alternatives.

• Quality Assurance: Global QMS with comprehensive and stringent QC release criteria.
• End-to-End GMP Compliance: Full manufacturing and QC under a cGMP system.
• Comprehensive Control of Adventitious Agents: Stringent biosafety from cell banks to final release.
• Comprehensive Regulatory Support: Includes RSF and DMF to meet global requirements.
• Resilient Supply Chain: Intelligent modular facilities ensure a stable global supply.
• Professional Support: Extensive manufacturing and application expertise to accelerate development.

GMP Human Betacellulin Protein (GMP-BENH15) is expressed from E. coli cells. It contains AA Asp 32 - Gln 118 (Accession # P35070-1).

Met-Asp-Gly-Asn-Ser-Thr-Arg-Ser-Pro-Glu-Thr-Asn-Gly-Leu-Leu
(Routinely tested)

Less than 10 EU/mg, tested by the rFC method in compliance with USP <86> and Ph. Eur. 2.6.32.

<0.5 ng/µg of protein tested by ELISA.

<0.02 ng/μg of protein tested by qPCR.

Sterility testing was performed using the membrane filtration method in compliance with USP <71> and Ph. Eur. 2.6.1.

Negative

>95% as determined by SDS-PAGE.

Lyophilized from 0.22 μm filtered solution in 20 mM Citric acid, pH2.2 with protectants.

Contact us for customized product form or formulation.

2R (13 mm neck finish)

This product is supplied and shipped on blue ice.

Upon receipt, store it immediately at -20°C or lower for long term storage.

Please avoid repeated freeze-thaw cycles.

• -20°C to -70°C for 5 years in lyophilized state;
• -70°C for 12 months under sterile conditions after reconstitution.