Alexa Fluor™ 647-Labeled Human Alpha-synuclein Protein, His Tag

AIDD‑Ready
Related Products: Cell line CHEK-ATP085
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ALN-HA1H3-200ug
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ALN-HA1H3-1mg
$3,150.00
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ALN-HA1H3
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Synonyms

SNCA, NACP, PARK1, alpha-Synuclein

Source

Alexa Fluor 647-Labeled Human Alpha-synuclein Protein, His Tag (ALN-HA1H3) is expressed from E. coli cells. It contains AA Met 1 - Ala 140 (Accession # P37840-1). It is the Alexa Fluor™ 647 labeled form of Human Alpha-Synuclein, His Tag (Cat. No. ALN-H52H8).

Predicted N-terminus: Met 1

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 16.3 kDa.

Conjugate

AF647

Excitation Wavelength: 640 nm

Emission Wavelength: 672 nm

Labeling

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF647 using standard chemical labeling method. The residual AF647 is removed by molecular sieve treatment during purification process.

Protein Ratio

The AF647 to protein molar ratio is 2-4.

Endotoxin

Less than 0.1 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • The product MUST be stored at -70°C or lower upon receipt;
  • -70°C for 3 months under sterile conditions.

バックグランド

Alpha-synuclein is a neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. It acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. Abnormalities in alpha-synuclein are implicated in the pathogenesis of Parkinson's disease (PD). Alpha-synuclein is present in Lewy-bodies, the neuropathological hallmark of PD, and the protein and its aggregation have been widely linked to neurotoxic pathways that ultimately lead to neurodegeneration.