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Cynomolgus Alpha-Synuclein Protein, His Tag (MALS verified)

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  • 分子别名(Synonym)

    SNCA,NACP,PARK1,alpha-Synuclein

  • 表达区间及表达系统(Source)

    Cynomolgus Alpha-Synuclein, His Tag (ALN-C52H5) is expressed from E. coli cells. It contains AA Met 1 - Ala 140 (Accession # P61142-1).

    Predicted N-terminus: Met 1

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    Alpha-Synuclein Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 16.4 kDa. The protein migrates as 18-20 kDa under reducing (R) condition (SDS-PAGE).

  • 偶联(Conjugate)

    Unconjugated

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method.

  • 纯度(Purity)

    95% as determined by SDS-PAGE.

    90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in 100 mM NaAC, pH7.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    Alpha-Synuclein SDS-PAGE

    Cynomolgus Alpha-Synuclein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    Alpha-Synuclein SEC-MALS

    The purity of Cynomolgus Alpha-Synuclein, His Tag (Cat. No. ALN-C52H5) is more than 90% and the molecular weight of this protein is around 14-20 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     Alpha-Synuclein ELISA

    Immobilized Cynomolgus Alpha-Synuclein, His Tag (Cat. No. ALN-C52H5) at 1 μg/mL (100 μL/well) can bind Anti-Synucelin alpha/beta Monoclonal Antibody with a linear range of 0.004-0.25 μg/mL (QC tested).

    Protocol

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背景介绍

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca2+ release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.

前沿进展

 
药物研发进展
  • 英文全称:

    Synuclein alpha

  • 中文全称:

    α-突触核蛋白

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    23 详情

  • 最高研发阶段:

    临床三期

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