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Activin E
Human Activin E Protein, His Tag (INE-H5143) is expressed from E. coli cells. It contains AA Thr 237 - Ser 350 (Accession # P58166).
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 14.5 kDa. The protein migrates as 15-16 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
The protein is designed as a dimer.
The molecular weight by mass spectrometry is 28951 Da (Routinely tested).
Human Activin E Protein, His Tag(INE-H5143) was digested with trypsin and characterized by LC-MS/MS peptide mapping, achieving 100% sequence coverage (Routinely tested).
Met-(Tag)-Thr-Pro-Thr-Cys-Glu-Pro-Ala-Thr-Pro-Leu-Cys-Cys-Arg-Arg-Asp
(Routinely tested)
Less than 0.01 EU per μg by the LAL method / rFC method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 20 mM Citric acid, pH2.2 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.