Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (For Serological Testing)

Based on the high binding affinity between biotin-streptavidin systems, our best seller SP-13 Streptavidin Coated Plates(For Serological Testing) are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.

The streptavidin coated plate is developed for immunoassay and ELISA of serum samples.

The unopened plate should be stored at 2°C to 8°C,The expiry date of the plate is 12 months. Once opened, place unused plates in a resealable bag with desiccant and store at 2°C to 8°C, The shelf life is 1 month from the date of opening.

Your experiment will include 5 simple steps:

a) Add your Biotinylated sample to the plate, incubation for 1 hour and wash the plate.

b) Add your sample to the plate, incubation for 1 hour and wash the plate.

c) Add a diluted Detection antibody to the plate and incubation.

d) Wash the plate and next add TMB or other colorimetric HRP substrate.

e) Stop the substrate reaction by add diluted acid, and Read OD at 450 nm, the OD Value reflects the amount of antibody bound.

Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated molecules can bind to the SA irreversibly. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. The Streptavidin Coated Plates we provide are easy to use and widely available for applications. Example ELISA Procedure.