Anti-MMAE Antibody Screening Panel

Antibody Internalization Detection Reagent (IGG-PZF2001) monitors endocytosis in live cells
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Product Details

This antibody panel comprises four distinct rabbit monoclonal antibodies against MMAE, with slight differences in their recognition epitopes. Consequently, they may exhibit varying affinity levels towards different MMAE analogs or different conjugates of MMAE and linkers. This panel can be used to conduct preliminary experiments for screening the optimal antibody clone.

Components
Cat.No.
Clone
Size
MME-MY2209
M1H09
20 ug
MME-MY2210
M1G04
20 ug
MME-MY2211
M1D12
20 ug
MME-MY2198a
M1H05
20 ug
Specificity

Specifically recognizes MMAE and does not recognize MMAF.

Source

This panel contains four anti-MMAE monoclonal antibodies, suitable for pharmacokinetic (PK) detection of ADC with MMAE as the payload. All the anti-MMAE monoclonal antibodies are recombinantly expressed from HEK293, can be used as a capture antibody for detecting conjugated antibodies in ELISA assay.

Isotype

Rabbit IgG, Kappa

Purification

Protein A purified.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background

Monomethyl auristatin E (MMAE) is a synthetic derivative of dolastatin 10 and functions as a potent mitotic inhibitor by inhibiting tubulin polymerization. MMAE is widely used as a cytotoxic component of antibody-drug conjugates (ADCs) to treat several cancer types. Anti-MMAE antibody is a rabbit monoclonal antibody. The anti-MMAE antibody is a useful reagent in PK assay to determine conjugated antibodies.