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Assay Type | Sandwich-ELISA |
Analyte | VH3 binding Protein A |
Format | 96T (8×12 strips) |
Regulatory Status | RUO |
Sensitivity | ＜50 pg/mL |
Standard Curve Range | 50 pg/mL-8100 pg/mL |
Assay Time | 2 h |
Suitable Sample Type | For the quantitative determination of recombinant VH3 binding protein A |
Sample volume | 50 μL |
VH3 ELISA Kit (Residue Testing) is based on Sandwich-ELISA and is used to measure both natural and recombinant forms of VH3 residues in antibody drug preparations, including structurally distinct variants such as MabSelect™ VH3. It is designed to provide an essential tool for optimal purification process development and routine quality control of in-process streams and final products, ensuring the accurate monitoring of VH3 levels and maintaining product quality.
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
ID | Components | Size |
RES059-C01 | Pre-Coated Anti-VH3 Antibody Microplate | 1 plate (8×12 strips) |
RES059-C02 | Recombinant VH3 binding Protein A Standard (1 μg/mL) | 100 μL |
RES059-C03 | Biotin-Anti-VH3 Antibody | 1.5 mL |
RES059-C04 | Streptavidin-HRP | 10μg |
RES059-C05 | 10×Sample Dilution Buffer | 15 mL |
RES059-C06 | Denaturation Buffer | 15 mL |
RES059-C07 | 20×Washing Buffer | 30 mL |
RES059-C08 | Antibody Dilution Buffer | 15 mL |
RES059-C09 | Streptavidin-HRP Dilution Buffer | 15 mL |
RES059-C10 | Substrate Solution | 12 mL |
RES059-C11 | Stop Solution | 8 mL |