Human VEGF ELISA Kit

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CEA-C068-96tests
$565.00
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CEA-C068
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Product Overview

Vascular endothelial growth factor (VEGF or VEGF-A) is a signaling protein that stimulates the formation of blood vessels (angiogenesis). There are several members of the VEGF family, with VEGF-A being the main and most extensively studied one. VEGF proteins function as homodimers (two identical subunits) linked by disulfide bonds. Each subunit is approximately 40–45 kDa, and the VEGF-A dimer binds to VEGF receptors (VEGFRs) on endothelial cells. Human VEGF ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human VEGF/VEGF-A dimers that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:

  • Standards to calibrate with NIBSC/WHO standards for comparable results.
  • Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  • High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  • Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay Type
Sandwich-ELISA
Analyte
VEGF
Format
96-wells plate breakable into 12 x 8 wells strips
Reactivity
Human
Sensitivity
<7 pg/mL
Assay Time
3 h 10 min
Sample volume
100 μL
Range
31.25 pg/mL-2000 pg/mL
Sample Type
Cell Culture Supernatants, Plasma, Serum.
NIBSC Code
19/246

Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

Application

This kit is specifically designed for the accurate quantification of human VEGF/VEGF-A dimers in cell culture supernatants, serum, and plasma. The standard used is a recombinant VEGF dimer protein, and the antibodies specifically recognize the VEGF dimer.

It is for research use only.

Workflow

VEGF-A Workflow

Storage

Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Materials Provided
ID
Components
Size
CEA068-C01
Pre-coated Anti-VEGF Antibody Microplate
1 plate
CEA068-C02
Human VEGF Standard
2000 pg×2
CEA068-C03
Biotin-Anti-VEGF Antibody Con. Solution
120 μL
CEA068-C04
Biotin-Antibody Dilution Buffer
15 mL
CEA068-C05
Streptavidin-HRP Con. Solution
120 μL
CEA068-C06
Streptavidin-HRP Dilution Buffer
15 mL
CEA068-C07
20×Washing Buffer
25 mL
CEA068-C08
Sample Dilution Buffer
15 mL×2
CEA068-C09
Substrate Solution
15 mL
CEA068-C10
Stop Solution
15 mL