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Vascular endothelial growth factor (VEGF or VEGF-A) is a signaling protein that stimulates the formation of blood vessels (angiogenesis). There are several members of the VEGF family, with VEGF-A being the main and most extensively studied one. VEGF proteins function as homodimers (two identical subunits) linked by disulfide bonds. Each subunit is approximately 40–45 kDa, and the VEGF-A dimer binds to VEGF receptors (VEGFRs) on endothelial cells. Human VEGF ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human VEGF/VEGF-A dimers that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
Assay Type | Sandwich-ELISA |
Analyte | VEGF |
Format | 96-wells plate breakable into 12 x 8 wells strips |
Reactivity | Human |
Sensitivity | <7 pg/mL |
Assay Time | 3 h 10 min |
Sample volume | 100 μL |
Range | 31.25 pg/mL-2000 pg/mL |
Sample Type | Cell Culture Supernatants, Plasma, Serum. |
NIBSC Code | 19/246 |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
This kit is specifically designed for the accurate quantification of human VEGF/VEGF-A dimers in cell culture supernatants, serum, and plasma. The standard used is a recombinant VEGF dimer protein, and the antibodies specifically recognize the VEGF dimer.
It is for research use only.

Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
ID | Components | Size |
CEA068-C01 | Pre-coated Anti-VEGF Antibody Microplate | 1 plate |
CEA068-C02 | Human VEGF Standard | 2000 pg×2 |
CEA068-C03 | Biotin-Anti-VEGF Antibody Con. Solution | 120 μL |
CEA068-C04 | Biotin-Antibody Dilution Buffer | 15 mL |
CEA068-C05 | Streptavidin-HRP Con. Solution | 120 μL |
CEA068-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
CEA068-C07 | 20×Washing Buffer | 25 mL |
CEA068-C08 | Sample Dilution Buffer | 15 mL×2 |
CEA068-C09 | Substrate Solution | 15 mL |
CEA068-C10 | Stop Solution | 15 mL |