Mouse TNF-alpha ELISA Kit

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CEA-M255-96tests
$565.00
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CEA-M255
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Product Overview

Mouse TNF-alpha ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant mouse TNF-alpha that is present in biological samples, such as serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:

  • Standards to calibrate with NIBSC/WHO standards for comparable results.
  • Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  • High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  • Ready-to-use reagents to save assay time.
Product Specifications
Assay Type
Sandwich-ELISA
Analyte
Mouse TNF-alpha
Format
96-wells plate breakable into 12 x 8 wells strips
Reactivity
Mouse
Sensitivity
4 pg/mL
Assay Time
2.75 h
Sample volume
50 μL
Range
7.81 pg/mL-500 pg/mL
Sample Type
For the quantitative determination of mouse TNF-alpha in serum, plasma and cell supernatant.
NIBSC Code
88/532, NIBSC/WHO (88/532) approximate value (U/mL) = 0.2 x ACRO Mouse TNF-α (pg/mL)

Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

Application

The kit is developed for quantitative detection of natural and recombinant mouseTNF-alpha in serum, plasma and cell culture supernatants.

It is for research use only.

Workflow

TNF-alpha Workflow

Storage

Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Materials Provided
ID
Components
Size
CEA255-C01
Pre-coated Anti-mouse TNF-alpha Antibody Microplate
1 plate
CEA255-C02
Mouse TNF-alpha Standard
5 ng×2
CEA255-C03
Biotin-Anti-mouse TNF-alpha Antibody Con. Solution
100 μL
CEA255-C04
Biotin-Antibody Dilution Buffer
8 mL
CEA255-C05
Streptavidin-HRP Con. Solution
500 μL
CEA255-C06
Streptavidin-HRP Dilution Buffer
15 mL
CEA255-C07
20×Washing Buffer
50 mL
CEA255-C08
Sample Dilution Buffer
15 mL×2
CEA255-C09
Substrate Solution
12 mL
CEA255-C10
Stop Solution
6 mL