Human Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit

Item number CRS-A002 has been upgraded to CEA-C246. We recommend purchasing it first!
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CRS-A002-96tests
$515.00
In Stock - Delivery in 2-3 business days
CRS-A002-480tests (96tests X 5)
$2,310.00
CRS-A002
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Product Overview

Human Tumor Necrosis Factor Alpha(TNF-α) ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human TNF-alpha that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:

  • Standards to calibrate with NIBSC/WHO standards for comparable results.
  • Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  • High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  • Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay Type
Sandwich-ELISA
Analyte
TNF-α
Format
96-wells plate breakable into 12 x 8 wells strips
Reactivity
Human
Sensitivity
2.321 pg/mL
Assay Time
2 h 50 min
Sample volume
100 μL
Range
19.5 pg/mL-1250 pg/mL
Sample Type
Cell Culture Supernatants, Plasma, Serum.

Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

Application

The kit is developed for quantitative detection of natural and recombinant human TNF-alpha in serum, plasma and cell culture supernatants.

It is for research use only.

Workflow

TNF-alpha Workflow

Storage

Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Materials Provided
ID
Components
Size
CRS002-C01
Pre-coated Anti-TNF-α Antibody Microplate
1 plate
CRS002-C02
Human TNF-α Standard
20 μg
CRS002-C03
Biotin-Anti-TNF-α Antibody
20 μg
CRS002-C04
Streptavidin-HRP
50 μL
CRS002-C05
10xWashing Buffer
50 mL
CRS002-C06
2xDilution Buffer
50 mL
CRS002-C07
Substrate Solution
12 mL
CRS002-C08
Stop Solution
7 mL