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产品概述（Product Details）
ClinMax™ Human Tumor Necrosis Factor Alpha(TNF-α) ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human TNF-alpha that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
应用说明（Application）
The kit is developed for quantitative detection of natural and recombinant human TNF-alpha in serum, plasma and cell culture supernatants.
It is for research use only.
流程图（Workflow）
关键信息（Key Features）
Assay TypeSandwich-ELISAAnalyteTNF-αFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity2.321 pg/mLAssay Time2 hr 50 minSample volume100 μLRange19.5 pg/mL-1250 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
存储（Storage）
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分（Materials Provided）
IDComponentsSizeCRS002-C01Pre-coated Anti-TNF-α Antibody Microplate1plateCRS002-C02Human TNF-α Standard20μgCRS002-C03Biotin-Anti-TNF-α Antibody20μgCRS002-C04Streptavidin-HRP50 μLCRS002-C0510xWashing Buffer50 mLCRS002-C062xDilution Buffer50 mLCRS002-C07Substrate Solution12 mLCRS002-C08Stop Solution7 mL质量管理控制体系（QMS）
产品展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of TNF-α is less than 2.321 pg/mL.
样本值（Sample Values）
Serum – Samples from apparently healthy volunteers were evaluated for the presence of human TNF-α in this assay. No medical histories were available for the donors used in this study.
Cell Culture Supernatant – Human peripheral blood mononuclear cells (PBMCs) were cultured and stimulated using CD28/OKT3 antibody then detected TNF-α secretion amount.
稀释线性（Dilution Linearity）
High concentrations (1200pg/mL) of human TNF-α were added to different diluted substrates for gradient dilution to evaluate the linearity of the assay.
批内差异（Intra-Assay Statistics）
20 replicates of each of 3 samples containing different TNF-α concentrations were tested in one assay. Acceptable criteria: CV＜15%.
批间差异（Inter-Assay Statistics）
3 samples containing 3 different concentrations of TNF-α were tested in independent assays. Acceptable criteria: CV<15%.
回收率（Recovery）
TNF-α was spiked into 3 human serum samples, and then analyzed. The average recovery of TNF-α for serum samples is 101.0%, range is 89.6% - 111.4%.
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