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Assay Type | Sandwich-ELISA |
Analyte | Protein A |
Format | 96T (8×12 strips) |
Regulatory Status | RUO |
Sensitivity | < 20 pg/mL |
Standard Curve Range | 25 pg/mL-1600 pg/mL |
Assay Time | 2 h |
Suitable Sample Type | For the quantitative determination of recombinant Protein A, alkaline-resistant Protein A and MaXtar® ARPA ligand Protein A. |
Sample volume | 50 μL |
Universal Protein A Quick ELISA kit is based on the ELISA sandwich method and is used to detect both natural and structurally conserved recombinant forms of Protein A, as well as recombinant variants with significant structural differences, such as MabSelect SuRe™, MaXtar® ARPA ligand (Bio-Link Co.), and others. It is designed to provides an essential tool for optimal purification process development and routine quality control of in-process streams and final products, ensuring accurate monitoring of Protein A levels and maintaining product quality.
Unopened kit should be stored at 2°C-8°C upon receiving.
Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
ID | Components | Size |
RES024-C01 | Pre-Coated Anti-Protein A Antibody Microplate | 1 plate (8×12 strips) |
RES024-C02A | Alkali-Tolerant Recombinant Protein A Standard (1 μg/mL) | 100 μL |
RES024-C02B | MaXtar® ARPA ligand Protein A Standard (Bio-Link Co.) (1 μg/mL) | 100 μL |
RES024-C03 | Recombinant Protein A Standard (1 μg/mL) | 100 μL |
RES024-C04 | Biotin-Anti-Protein A Antibody | 700 μL |
RES024-C05 | Streptavidin-HRP | 300 μL |
RES024-C06 | Dilution Buffer | 100 mL |
RES024-C07 | 20×Washing Buffer | 30 mL |
RES024-C08 | Substrate Solution | 12 mL |
RES024-C09 | Stop Solution | 6 mL |