pH-Sensitive Fluorescent Dye Antibody Labeling Kit (Em660, 100μg/Reaction)

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ALK-A004-3Reactions
$450.00
In Stock - Delivery in 2-3 business days
ALK-A004
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FAB
  • Direct labeling - Eliminates secondary antibodies for a more direct signal, smaller molecular weight, and greater analytical flexibility.
  • High signal to noise ratio - Strong fluorescence with minimal background.
  • pH sensitive - Bright emission in acidic milieu.
  • Rapid fluorescent labeling - Only 30 minutes of hands-on time.
  • Broad antibody compatibility​ - Labeling via antibody amines works across different antibody species and subtypes.
Product Overview

The pH-sensitive dye (Em660) cannot only significantly enhance the fluorescence signal in an acidic environment but also minimize background noise effectively, which has an excitation wavelength of 643 nm and an emission wavelength of 660 nm.

pH-Sensitive Fluorescent Dye Antibody Labeling Kit provides a rapid and simple method to covalently label antibodies with pH-sensitive fluorescent dye by targeting primary amine groups.

Application

pH-sensitive Dye Antibody Labeling Kit (Em660) provides a rapid and simple method for achieving covalent coupling between pH-sensitive fluorescent dye and antibody molecules. The conjugation reaction requires simple purification via spin column chromatography with straightforward operation, and the prepared dye-labeled antibody can be used for the detection of antibody internalization efficiency in cells.

It is for research use only.

Storage

The transport temperature for the kit is 2-8°C.

• BoxA: Unopened kit should be stored at -20℃ upon receiving.

• BoxB: Unopened kit should be stored at 2-8℃ upon receiving.

Find the expiration date on the outside packaging and do not use reagents past their expiration date.

Assay Principles

pH-sensitive fluorescent dye Assay Principles

BoxA(-20°C)
ID
Components
Size
ALK-A004-C01
Activated pH-Sensitive Fluorescent Dye (Em660)
1 vialx3
ALK-A004-C02
1M NaHCO3
1 vial
ALK-A004-C03
Quencher Reagent
1 vial
BoxB(4°C)
ID
Components
Size
ALK-A004-1-C01
1xPBS (pH7.2-7.4), 11% Trehalose
5 mL
ALK-A004-1-C02
Desalting Spin column 0.5 mL
3 per