Human Perforin ELISA Kit

製品番号/仕様
価格
Availability
数量
CEA-B034-96tests
$515.00
CEA-B034
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Product Overview

Human Perforin ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human Perforin that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:

  • Standards to calibrate with NIBSC/WHO standards for comparable results.
  • Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  • High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  • Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay Type
Sandwich-ELISA
Analyte
Perforin
Format
96-wells plate breakable into 12 x 8 wells strips
Reactivity
Human
Sensitivity
60 pg/mL
Assay Time
2.75 h
Sample volume
100 μL
Range
62.5 pg/mL-2000 pg/mL
Sample Type
Cell Culture Supernatants, Plasma, Serum.

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Application

The kit is developed for quantitative detection of natural and recombinant human Perforin in serum, plasma and cell culture supernatants.

It is for research use only.

Workflow

Perforin Workflow

Storage

Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Materials Provided
ID
Components
Size
CEA034-C01
Pre-coated Anti-Perforin Antibody Microplate
1 plate
CEA034-C02
Human Perforin Standard
1ng×2
CEA034-C03
Biotin-Anti-Perforin Antibody Con. Solution
150 μL
CEA034-C04
Biotin-Antibody Dilution Buffer
15 mL
CEA034-C05
Streptavidin-HRP Con. Solution
1.5 mL
CEA034-C06
Streptavidin-HRP Dilution Buffer
15 mL
CEA034-C07
20×Washing Buffer
50 mL
CEA034-C08
Sample Dilution Buffer
15 mL×2
CEA034-C09
Substrate Solution
12 mL
CEA034-C10
Stop Solution
6 mL