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Human Laminin Quantitative ELISA Kit

COAMS
DS
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  • 产品参数（Product Specifications）

    Assay Type
    Sandwich-ELISA
    Analyte
    Laminin
    Format
    96T(8×12 strips)
    Reactivity
    Human
    Regulatory Status
    RUO
    Sensitivity
    ＜3.125 ng/mL
    Standard Curve Range
    3.125 ng/mL-100 ng/mL
    Assay Time
    3 hr 20 min
    Suitable Sample Type
    For the quantitative determination of Laminin in human serum and cell culture supernates.
    Sample volume
    100 uL

  • 产品概述（Product Overview）

    Human Laminin Quantitative ELISA Kit is based on the Sandwich-ELISA method and is used to detect and quantitatively measure Laminin, including the specific identification of full-length Laminin 521, Laminin 521 E8 fragment, full-length Laminin 511, and Laminin 511 E8 fragment levels, in cell culture supernatants, serum, and plasma. It is designed to provide rigorous quality control, helping to monitor Laminin levels in biological samples and contribute to process optimization in the production of cell-based therapies.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

  • 存储（Storage）

    2-8℃

  • 组分（Materials Provided）

    ID
    Components
    Size
    RES091-C01
    Pre-coated Anti-Laminin Antibody Microplate
    1 plate(8×12 strips)
    RES091-C02
    Human Laminin 521 Standard
    20 μg
    RES091-C03
    Biotin-Anti-Laminin Antibody
    20 μg
    RES091-C04
    Streptavidin-HRP
    50 μL
    RES091-C05
    10×Washing Buffer
    50 mL
    RES091-C06
    2×Dilution Buffer
    50 mL
    RES091-C07
    Substrate Solution
    12 mL
    RES091-C08
    Stop Solution
    7 mL

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

产品展示

  • 典型数据-Typical Data

    Please refer to DS document for the assay protocol.

    Laminin TYPICAL DATA

    For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

验证

  • 稀释线性（Dilution Linearity）

    To assess the linearity of the assay, samples spiked with high concentrations of Laminin 521 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

    Laminin DILUTION LINEARITY

  • 批内差异（Intra-Assay Statistics）

    Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.

    Laminin INTRA-ASSAY STATISTICS

  • 批间差异（Inter-Assay Statistics）

    Three samples of known concentration were tested in three separate assays to assess inter-assay precision.

    Laminin INTER-ASSAY STATISTICS

  • 回收率（Recovery）

    Three Laminin 521 with different concentrations were tested to calculate the recovery rate.

    Laminin RECOVERY

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背景介绍

Laminin is a class of Basement membrane (BM) proteins, Laminin affects cell adhesion, migration phenotypic maintenance, cell survival, and cell differentiation, Laminin is an isomeric trimer with different kinds of chains, five kinds of α chains, four kinds of β chains, and three kinds of γ chains can form more than 60 combinations. Among them, LN511 and LN521 are the ubiquitous BM protein components, they play important roles in the development of differentiation scheme.

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