Human IL-4 / IL-4RA Inhibitor Screening Kit (TR-FRET)

• Simple and fast operation - No complicated washing steps, significantly reducing time.
• High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.
• Accurate and reliable results - High sensitivity with minimal matrix effects.
• High throughput capability - Support multiple product specifications, ideal for high-throughput screening.
• Fast completion - Results in just 1 hour.

The Human IL-4 / IL-4RA Inhibitor Screening Kit (TR-FRET) is based on a homogeneous (no wash) competition TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer) to screening for inhibitors of human IL-4 binding to human IL-4RA within 0.5-1 hours. It can also be used as a universal detection tool to identify the ability of human IL-4 to bind to human IL-4RA.

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

This Human IL-4 / IL-4RA Inhibitor Screening Kit (TR-FRET) is based on TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer). This assay uses the mixture of biotinylated human IL-4 and Europium-chelate labeled streptavidin as the Donor and FA Labeled Human IL-4RA Protein as the Acceptor.

— When the sample does not contain the inhibitors that block the binding of human IL-4 to human IL-4RA, the Donor and Acceptor are in close proximity because of the binding of human IL-4 and human IL-4RA. Upon Donor excitation with light of a specific wavelength (337nm), in addition to Donor emission (620nm), non-radiative transfer of energy occurs between Donor and Acceptor, resulting in Acceptor emission (665nm).

— When the sample contains the inhibitors that block the binding of human IL-4 to human IL-4RA, the components inhibit the binding between the Donor and Acceptor and thereby prevent FRET from occurring.