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Assay Type | Sandwich-ELISA |
Analyte | IL-10 |
Format | 96T (8×12 strips) |
Reactivity | Human |
Regulatory Status | RUO |
Sensitivity | ＜6.25 pg/mL |
Standard Curve Range | 6.25 pg/mL-200 pg/mL |
Assay Time | 2 h 50 min |
Suitable Sample Type | For detection and quantitative determination of GMP human IL-10 in samples from CAR-T product preparation processing. |
Sample volume | 100 μL |
Human Interleukin-10 (IL-10) ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine GMP human IL-10 in cell culture supernatants, serum, and plasma. The kit contains GMP human IL-10 (ACROBiosystems, cat#GMP-L10H25) to ensure accurate assay results. It is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. Additionally, it can be used as a universal detection tool for the quantitative determination of human IL-10.
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
ID | Components | Size |
CRS008-C01 | Pre-coated Anti-IL-10 Antibody Microplate | 1 plate (8×12 strips) |
CRS008-C02 | Human IL-10 Standard | 20 μg |
CRS008-C03 | Biotin-Anti-IL-10 Antibody | 50 μL |
CRS008-C04 | Streptavidin-HRP | 50 μL |
CRS008-C05 | 10×Washing Buffer | 50 mL |
CRS008-C06 | 2×Dilution Buffer | 50 mL |
CRS008-C07 | Substrate Solution | 12 mL |
CRS008-C08 | Stop Solution | 7 mL |