产品详情
产品概述(Product Details)
ClinMax™ Human IL-1β ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IL-1β that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ClinMax™ ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
应用说明(Application)
The kit is developed for quantitative detection of natural and recombinant human IL-1β in serum, plasma and cell culture supernatants.
It is for research use only.
流程图(Workflow)
关键信息(Key Features)
Assay TypeSandwich-ELISAAnalyteIL-1βFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity1.0 pg/mLAssay Time1.75 hrSample volume50 uLRange4.69 pg/mL-600 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.NIBSC Code86/680Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
存储(Storage)
Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
组分(Materials Provided)
IDComponentsSizeCRB002-C01Pre-coated Anti-IL-1β Antibody Microplate1 plateCRB002-C02Human IL-1β Standard20 μg×2CRB002-C03Biotin-Anti-IL-1β Antibody Con. Solution100 μLCRB002-C04Biotin-Antibody Dilution Buffer8 mLCRB002-C05Streptavidin-HRP Con. Solution500 μLCRB002-C06Streptavidin-HRP Dilution Buffer15 mLCRB002-C0720× Washing Buffer50 mLCRB002-C081× Dilution Buffer15 mL×2CRB002-C09Substrate Solution12 mLCRB002-C10Stop Solution6 mL质量管理控制体系(QMS)
数据展示
典型数据-Typical Data
Please refer to DS document for the assay protocol.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CRS-B002 is less than 1.0 pg/mL.
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