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Assay Type | Sandwich-ELISA |
Analyte | IL-1 beta |
Format | 96T (8×12 strips) |
Reactivity | Human |
Regulatory Status | RUO |
Sensitivity | <4.688 pg/mL |
Standard Curve Range | 4.688 pg/mL-150 pg/mL |
Assay Time | 2 h 50 min |
Suitable Sample Type | For the detection and quantitative determination of IL-1 beta in human serum and cell culture supernatant. |
Sample volume | 100 μL |
Human Interleukin-1 Beta (IL-1 Beta) ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine GMP human IL-1 beta in cell culture supernatants, serum, and plasma. The kit contains GMP human IL-1 beta (ACROBiosystems, cat#GMP-ILBH16) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of human IL-1 beta.
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
ID | Components | Size |
CRS012-C01 | Pre-coated Anti-IL-1 beta Antibody Microplate | 1 plate (8×12 strips) |
CRS012-C02 | Human IL-1 beta Standard | 20 μg |
CRS012-C03 | Biotin-Anti-IL-1 beta Antibody | 20 μg |
CRS012-C04 | Streptavidin-HRP | 50 μL |
CRS012-C05 | 10×Washing Buffer | 50 mL |
CRS012-C06 | 2×Dilution Buffer | 50 mL |
CRS012-C07 | Substrate Solution | 12 mL |
CRS012-C08 | Stop Solution | 7 mL |