Human Fc Detection Kit (TR-FRET)

• Comprehensive validation - Rigorously validated through comprehensive methodology to ensure reliable and accurate results.
• Simple and fast operation - No complicated washing steps, significantly reducing time.
• High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.
• Accurate and reliable results - High sensitivity with minimal matrix effects.
• High throughput capability - Support multiple product specifications, ideal for high-throughput screening.
• Fast completion - Results in just 1 hour.

Human Fc Detection Kit (TR-FRET) is based on a homogeneous (no wash) competition TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer) to quantify human IgG or human Fc-tagged proteins within 0.5-1 hours. The kit is compatible with both natural and recombinant human IgG.

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

This Human Fc Detection Kit (TR-FRET) is based on TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer). This assay uses Anti-Human IgG Fc Antibody Europium-chelate as the Donor and FA Labeled Human IgG Antibody as the Acceptor.

— When the sample does not contain human IgG or human Fc-tagged proteins, the Donor and Acceptor are in close proximity because of the binding of Anti-Human IgG Fc Antibody Europium-chelate and FA Labeled Human IgG Antibody. Upon Donor excitation with light of a specific wavelength (337nm), in addition to Donor emission (620nm), non-radiative transfer of energy occurs between Donor and Acceptor, resulting in Acceptor emission (665nm).

— When the sample contains human IgG or human Fc-tagged proteins, the components inhibit the binding between the Donor and Acceptor and thereby prevent FRET from occurring.