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Assay Type | Sandwich-ELISA |
Analyte | IFN-γ |
Format | 96T (8×12 strips) |
Reactivity | Human |
Regulatory Status | RUO |
Sensitivity | < 3.125 pg/mL |
Standard Curve Range | 3.125-200 pg/mL |
Assay Time | 2 h 50 min |
Suitable Sample Type | For the quantitative determination of human IFN-γ in Cell Culture Supernatants, Plasma, Serum. |
Sample volume | 100 μL |
Human Interferon-γ (IFN-γ) ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine GMP human IFN-γ residues in cell culture supernatants, serum, and plasma. The kit contains GMP Human Interferon-γ (IFN-γ) (ACROBiosystems, cat# GMP-IFGH24) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of Human IFN-γ.
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
ID | Components | Size |
RES028-C01 | Pre-coated Anti-IFN-γ Antibody Microplate | 1 plate (8×12 strips) |
RES028-C02 | Human IFN-γ Standard | 20 μg |
RES028-C03 | Biotin-Anti-IFN-γ Antibody | 50 μL |
RES028-C04 | Streptavidin-HRP | 50 μL |
RES028-C05 | 10xWashing Buffer | 50 mL |
RES028-C06 | 2xDilution Buffer | 50 mL |
RES028-C07 | Substrate Solution | 12 mL |
RES028-C08 | Stop Solution | 7 mL |