Human HGF ELISA Kit

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CRS-B012-96tests
$565.00
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CRS-B012-480tests (96tests X 5)
$2,545.00
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Product Overview

Human HGF ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human HGF that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:

  • Standards to calibrate with NIBSC/WHO standards for comparable results.
  • Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  • High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  • Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay Type
Sandwich-ELISA
Analyte
HGF
Format
96-wells plate breakable into 12 x 8 wells strips
Reactivity
Human
Sensitivity
40 pg/mL
Assay Time
1 h 45 min
Sample volume
50 μL
Range
62.5 pg/mL-8000 pg/mL
Sample Type
Cell Culture Supernatants, Plasma, Serum.
NIBSC Code
96/564

Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

Application

The kit is developed for quantitative detection of natural and recombinant human HGF in serum, plasma and cell culture supernatants.

It is for research use only.

Workflow

HGF Workflow

Storage

Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Materials Provided
ID
Components
Size
CRB012-C01
Pre-coated Anti-HGF Antibody Microplate
1 plate
CRB012-C02
Human HGF Standard
20 μg×2
CRB012-C03
Biotin-Anti-HGF Antibody Con. Solution
100 μL
CRB012-C04
Biotin-Antibody Dilution Buffer
8 mL
CRB012-C05
Streptavidin-HRP Con. Solution
500 μL
CRB012-C06
Streptavidin-HRP Dilution Buffer
15 mL
CRB012-C07
20×Washing Buffer
50 mL
CRB012-C08
1×Dilution Buffer
15 mL×2
CRB012-C09
Substrate Solution
12 mL
CRB012-C10
Stop Solution
6 mL