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Assay Type | Sandwich-ELISA |
Analyte | Fibronectin |
Format | 96T (8×12 strips) |
Reactivity | Human |
Regulatory Status | RUO |
Sensitivity | <100 pg/mL |
Standard Curve Range | 100 pg/mL-3200 pg/mL |
Assay Time | 3 h 20 min |
Suitable Sample Type | For the detection and quantitative determination of Human Fibronectin in cell culture supernates. |
Sample volume | 100 uL |
Fibronectin, a vital extracellular matrix protein, significantly impacts cell adhesion, spreading, migration, tissue repair, cell shape maintenance, and cell signaling. It exists as a dimer with disulfide - linked polypeptide chains and multiple functional domains. Different isoforms, like soluble and cell - associated ones, play distinct roles in wound healing, blood clotting, and cell - matrix interactions.
To support the development of cell therapy drugs, ACROBiosystems developed Human fibronectin quantitive ELISA Kit with rigorous methodological validation, which is used for the detection and quantitative determination of fibronectin in cell culture supernates, serum, and plasma. Besides, this kit can also be used for the quantitative determination of Recombinant Fibronectin fragment, premium grade (ACROBiosystems, cat# GMP-FINH18) concentrations.
The kit is developed for the detection and quantitative determination of fibronectin in cell culture supernates.
It is for research use only.
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
ID | Components | Size |
RES108-C01 | Pre-coated Anti-Human Fibronectin Antibody Microplate | 1 plate(8×12 strips) |
RES108-C02 | Human Fibronectin Standard | 5 μg×2 |
RES108-C03 | Biotin-Anti-Human Fibronectin Antibody | 20 μg |
RES108-C04 | Streptavidin-HRP | 50 μL |
RES108-C05 | 10×Washing Buffer | 50 mL |
RES108-C06 | 2×Dilution Buffer | 50 mL |
RES108-C07 | Substrate Solution | 12 mL |
RES108-C08 | Stop Solution | 7 mL |