Human Fc gamma RIIA / CD32a (H167) binding Kit (TR-FRET)

AIDD‑Ready
Should you require additional buffer, you can place a separate order.
製品番号/仕様
価格
Availability
数量
FRT-04-100tests
$860.00
In Stock - Delivery in 2-3 business days
FRT-04-500tests
$2,875.00
FRT-04
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FAB
  • Comprehensive validation - Validated with various antibody subtypes and antibody drugs.
  • Simple and fast operation - No complicated washing steps, significantly reducing time.
  • High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.
  • Accurate and reliable results - High sensitivity with minimal matrix effects.
  • High throughput capability - Support multiple product specifications, ideal for high-throughput screening.
  • Fast completion - Results in just 1 hour.
Product Specifications

Assay Type
Competition-TR-FRET
Reactivity
Human
Regulatory Status
RUO
Assay Time
1 h
Sample volume
10 μL

Product Overview

The Human Fc gamma RIIA / CD32a (H167) binding Kit (TR-FRET) is based on a homogeneous (no wash) competition TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer) to measure the interaction between human CD32a (H167) and antibody drug candidates or CD32a (H167) inhibitors. It is designed to facilitate the ADCC and ADCP functional performance evaluation of antibody drug candidates, high-throughput screening of CD32a (H167) inhibitors within 0.5-1 hours. It can also be used as a universal detection tool to identify the ability of antibody drugs to bind to human CD32a (H167).

Storage

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Materials Provided

ID
Components
Size
FRT04-C01
Human Fc gamma RIIA / CD32a (H167) Protein Europium-chelate
100 tests/500 tests
FRT04-C02
FA labeled human IgG antibody
100 tests/500 tests
FRT04-C03
Human IgG Standard
400 μg/100 tests
2 mg/500 tests
DB-04
TR-FRET Sample Dilution Buffer, pH7.4
50 mL/100 tests & 500 tests
DB-05
TR-FRET Detection Buffer, pH7.4
50 mL/100 tests & 500 tests

Assay Principles

This Human Fc gamma RIIA / CD32a (H167) binding Kit (TR-FRET) is based on TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer). This assay uses the mixture of biotinylated human Fc gamma RIIA / CD32a (H167) and Europium-chelate labeled streptavidin as the Donor and FA labeled human IgG antibody as the Acceptor.

Assay Principles