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Assay Type | Competition-TR-FRET |
Reactivity | Human |
Regulatory Status | RUO |
Assay Time | 1 h |
Sample volume | 10 μL |
The Human Fc gamma RIIA / CD32a (H167) binding Kit (TR-FRET) is based on a homogeneous (no wash) competition TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer) to measure the interaction between human CD32a (H167) and antibody drug candidates or CD32a (H167) inhibitors. It is designed to facilitate the ADCC and ADCP functional performance evaluation of antibody drug candidates, high-throughput screening of CD32a (H167) inhibitors within 0.5-1 hours. It can also be used as a universal detection tool to identify the ability of antibody drugs to bind to human CD32a (H167).
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
ID | Components | Size |
FRT04-C01 | Human Fc gamma RIIA / CD32a (H167) Protein Europium-chelate | 100 tests/500 tests |
FRT04-C02 | FA labeled human IgG antibody | 100 tests/500 tests |
FRT04-C03 | Human IgG Standard | 400 μg/100 tests 2 mg/500 tests |
DB-04 | TR-FRET Sample Dilution Buffer, pH7.4 | 50 mL/100 tests & 500 tests |
DB-05 | TR-FRET Detection Buffer, pH7.4 | 50 mL/100 tests & 500 tests |
This Human Fc gamma RIIA / CD32a (H167) binding Kit (TR-FRET) is based on TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer). This assay uses the mixture of biotinylated human Fc gamma RIIA / CD32a (H167) and Europium-chelate labeled streptavidin as the Donor and FA labeled human IgG antibody as the Acceptor.
