Human Complement C5 ELISA Kit

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CEA-B241-96tests
$565.00
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CEA-B241
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Product Overview

Human Complement C5 ELISA Kit ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantify natural and recombinant human Complement C5 (not C5a) that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:

  • Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  • High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  • Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay Type
Sandwich-ELISA
Analyte
Complement C5
Format
96-wells plate breakable into 12 x 8 wells strips
Reactivity
Human
Sensitivity
40 pg/mL
Assay Time
2.75 h
Sample volume
50 μL
Range
78.125 pg/mL-5000 pg/mL
Sample Type
For the quantitative determination of human Complement C5 in serum, plasma and cell supernatant.

Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

Application

The kit is developed for quantitative detection of human complement C5 in serum, plasma and cell culture supernates.

It is for research use only.

Workflow

Complement C5 Workflow

Storage

Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Materials Provided
ID
Components
Size
CEA241-C01
Pre-coated Anti-Complement C5 Antibody Microplate
1 plate
CEA241-C02
Human Complement C5 Standard
1 μg×2
CEA241-C03
Biotin-Anti-Complement C5 Antibody Con. Solution
100 μL
CEA241-C04
Biotin-Antibody Dilution Buffer
8 mL
CEA241-C05
Streptavidin-HRP Con. Solution
500 μL
CEA241-C06
Streptavidin-HRP Dilution Buffer
15 mL
CEA241-C07
20× Washing Buffer
50 mL
CEA241-C08
Sample Dilution Buffer
15 mL×2
CEA241-C09
Substrate Solution
12 mL
CEA241-C10
Stop Solution
6 mL