Human Activin E Dimer ELISA Kit

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CEA-B247-96tests
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Product Overview

Activin E is a key member of the transforming growth factor-β (TGF-β) superfamily. Primarily encoded by the INHBE gene and highly expressed in the liver, it is a dimeric protein (≈23 kDa) formed by two βE subunits linked via disulfide bonds. This kit is specifically designed for the accurate quantitation of mature human Activin E Dimer from cell culture supernates, serum and plasma. The standard is a recombinant mature Activin E dimer protein, and the antibodies specifically recognize the mature Activin E dimer. Our ELISA Kit provides several benefits:

  • Dimer-specific antibody pair: Detects only active Activin E dimer, no cross-reactivity with Oligomeric/non-dimeric forms.
  • Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
  • High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
  • Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay Type
Sandwich-ELISA
Analyte
Activin E Dimer
Format
96-wells plate breakable into 12 x 8 wells strips
Reactivity
Human/NHP/Mouse
Sensitivity
60 pg/ml
Assay Time
2.75 h
Sample volume
50 μL
Range
78.125 pg/mL-5000 pg/mL
Sample Type
For the quantitative determination human/NHP/mouse Activin E Dimer in serum, plasma and cell superatant.

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Application

The kit is developed for quantitative detection of natural and recombinant mature human/NHP/mouse Activin E dimer (not monomeric INHBE) in serum, plasma and cell culture supernates.

It is for research use only.

Workflow

Activin E Workflow

Storage

Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Materials Provided
ID
Components
Size
CEA247-C01
Pre-coated Anti-Activin E Dimer Antibody Microplate
1 plate
CEA247-C02
Human Activin E Dimer Standard
1.8 μg×2
CEA247-C03
Biotin-Anti-Activin E Dimer Antibody Con. Solution
100 μL
CEA247-C04
Biotin-Antibody Dilution Buffer
8 mL
CEA247-C05
Streptavidin-HRP Con. Solution
500 μL
CEA247-C06
Streptavidin-HRP Dilution Buffer
15 mL
CEA247-C07
20×Washing Buffer
50 mL
CEA247-C08
Sample Dilution Buffer
15 mL×2
CEA247-C09
Substrate Solution
12 mL
CEA247-C10
Stop Solution
6 mL