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Assay Type | Sandwich-ELISA |
Analyte | 4-1BB Ligand |
Format | 96T (8×12 strips) |
Reactivity | Human |
Regulatory Status | RUO |
Sensitivity | ＜15.625 pg/mL |
Standard Curve Range | 15.625 pg/mL-500 pg/mL |
Assay Time | 2 h 50 min |
Suitable Sample Type | For the detection and quantitative determination of 4-1BB Ligand in human serum and cell culture supernatant. |
Sample volume | 100 μL |
Human 4-1BB Ligand ELISA Kit (Residue Testing) is based on the ELISA sandwich method and is used to detect and quantitatively determine GMP human 4-1BB Ligand residues in cell culture supernatants, serum, and plasma. The kit contains GMP human 4-1BB Ligand (ACROBiosystems, cat# GMP-41LH26) to ensure accurate assay results and is designed to provide a reliable solution for CAR-T product quality assessment during drug development and CMC quality control stages. It can also be used as a universal detection tool for the quantitative determination of Human 4-1BB Ligand.
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
ID | Components | Size |
RES036-C01 | Pre-coated Anti-4-1BB Ligand Antibody Microplate | 1 plate (8×12 strips) |
RES036-C02 | Human 4-1BB Ligand Standard | 20 μg |
RES036-C03 | Biotin-Anti-4-1BB Ligand Antibody | 20 μg |
RES036-C04 | Streptavidin-HRP | 50 μL |
RES036-C05 | 10×Washing Buffer | 50 mL |
RES036-C06 | 2×Dilution Buffer | 50 mL |
RES036-C07 | Substrate Solution | 12 mL |
RES036-C08 | Stop Solution | 7 mL |