HEK293/Membrane-Bound human TL1A Stable Cell Line

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The HEK293/Membrane-Bound Human TL1A Stable Cell Line was engineered to express the only membrane-bound human TL1A by deletion of the amino acids 66–94 in full-length human TL1A (Uniprot: O95150-1), which was in loss of detectable membrane-bound human TL1A in the supernatant. Surface expression of the membrane-bound TL1A was confirmed by flow cytometry.

• Useful for cell-based TL1A binding assay

Adherent

Puromycin (2 μg/mL)

DMEM + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.