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HEK293/Human ROR1 Stable Cell Line

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CHEK-ATP084-2Vials (1Vial X 2)
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产品详情

    1. Genetically modified cell lines best reflect MOA (Mechanism of Action)
    2. Higher activity and larger assay window for robust and reproducible cell-based bioassay
    3. Comprehensive application data to support assay development and validation
    4. Full tracible record, stringent quality control and validated cell passage stability
    5. Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
    6. Global commercial license assistance whenever regulatory filing is required

  • 描述（Description）

    The HEK293/Human ROR1 Stable Cell Line was engineered to express the receptor full length human ROR1 (Uniprot: Q01973-1), used to mimic cancer target cells. Surface expression of Human ROR1 was confirmed by flow cytometry.

  • 应用说明（Application）

    • Useful for cell-based ROR1 binding assay

  • 生长特性（Growth Properties）

    Adherent

  • 筛选标记（Selection Marker）

    Puromycin (2 μg/mL)

  • 培养基（Complete Growth Medium）

    DMEM medium + 10% FBS

  • 冻存液（Freeze Medium）

    Serum-free cell cryopreservation medium

  • 装量（Quantity）

    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

  • 存储（Storage）

    Frozen in liquid nitrogen.

  • 支原体检测（Mycoplasma Testing）

    Negative

  • 无菌检测（Sterility Testing）

    Negative

  • 使用说明（Instructions for Use）

    See data sheet for detailed culturing and assay protocol.

产品展示

  • Receptor Assay

    ROR1 FACS

    Expression analysis of human ROR1 on HEK293/Human ROR1 Stable Cell Line by FACS.
    Cell surface staining was performed on HEK293/Human ROR1 Stable Cell Line or negative control cell using PE-labeled anti-human ROR1 antibody.

    Protocol
*如有相关细胞池需求请联系我们

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背景介绍

Tyrosine-protein kinase transmembrane receptor ROR1 is also known as Neurotrophic tyrosine kinase, receptor-related 1 (NTRKR1), which belongs to the protein kinase superfamily or tyr protein kinase family or ROR subfamily. ROR1 contains 1 FZ (frizzled) domain, 1 Ig-like C2-type (immunoglobulin-like) domain, 1 kringle domain, 1 protein kinase domain. ROR1 is expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. ROR1 could interact with casein kinase 1 epsilon (CK1ε) to activate phosphoinositide 3-kinase-mediated AKT phosphorylation and cAMP-response-element-binding protein (CREB), which was associated with enhanced tumor-cell growth.

前沿进展

 
药物研发进展
  • 英文全称：

    Inactive tyrosine-protein kinase transmembrane receptor ROR1

  • 中文全称：

    受体酪氨酸激酶样孤儿受体1

  • 种类：

  • 上市药物数量：

    0 详情

  • 临床药物数量：

    13 详情

  • 最高研发阶段：

    临床三期

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