Human PTH1R (Luc) HEK293 Reporter Cell

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The Human PTH1R (Luc) HEK293 Reporter Cell was engineered to not only express CREB signaling response element, but also express the receptor full length human PTH1R (Uniprot: Q03431), which can drive luciferase expressing systems by PTH1R agonists or PTH stimulation. In the absence of agonist or PTH, the PTH1R receptor is not activated and luminescence signal is low. In the presence of agonist or PTH, the PTH1R pathway-activated luminescence can be detected in a dose-dependent manner.

• Screen for agonists that can bind and activate PTH1R

Adherent

Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL)

DMEM + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.