HEK293/Human PD-L1, GFP Tag Stable Cell Line

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CHEK-ATP002-2Vials (1Vial X 2)
$4,785.00
CHEK-ATP002
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Advantages
  • Genetically modified cell lines best reflect MOA (Mechanism of Action)
  • Higher activity and larger assay window for robust and reproducible cell-based bioassay
  • Comprehensive application data to support assay development and validation
  • Full tracible record, stringent quality control and validated cell passage stability
  • Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
  • Global commercial license assistance whenever regulatory filing is required
Description

Human PD-L1, GFP Tag-HEK293 cells stably expressing exogenous human PD-L1 (Programmed cell death 1 ligand 1, CD274, B7-H1, PDCD1L1, PDCD1LG1, AA sequence: Leu 19 - Leu 238, Protein Acc. #: Q9NZQ7-1). At the same time, GFP protein as a marker was expressed in HEK239/hPD-L1 cells for screening positive cells by FACS.

Application

• Useful for cell-based PD-L1 binding assay.

Growth Properties

Adherent

Selection Marker

G418 (500 μg/mL)

Complete Growth Medium

DMEM + 10% FBS

Freeze Medium

Serum-free cell cryopreservation medium

Quantity

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Storage

Frozen in liquid nitrogen.

Mycoplasma Testing

Negative

Sterility Testing

Negative

Instructions for Use

See data sheet for detailed culturing and assay protocol.