Human IL-4 R alpha & Glucocorticoid Receptor (Luc) HEK293 Reporter Cell

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The Human IL-4 R alpha & Glucocorticoid Receptor (Luc) HEK293 Reporter Cell expressing human glucocorticoid receptor (GR) was engineered to not only express the glucocorticoid response element (GRE), but also express the receptor full length human IL-4 R alpha (Uniprot: P24394-1). In the absence of the IL-4 R alpha-ADC (via an anti-IL-4 R alpha antibody conjugated to a glucocorticoid agonist to form an anti-IL-4 R alpha antibody-drug conjugate), the human GR is not activated and luminescence signal is low. In the presence of the IL-4 R alpha-ADC, the glucocorticoid agonist released from the IL-4 R alpha-ADC can bind the human GR, resulting in the GRE-mediated luminescence.

• Screen for the IL-4 R alpha-targeting antibody-drug conjugate (IL-4 R alpha-ADC), formed by conjugating an anti-IL-4 R alpha antibody with a glucocorticoid agonist

Adherent

Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL)

DMEM + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.