Human IL-21 R/CD132 (Luc) HEK293 Reporter Cell

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The Human IL-21 R/CD132 (Luc) HEK293 Reporter Cell was engineered to not only express STAT3 signaling response element, but also express the receptors full length human IL-21 R (Uniprot: Q9HBE5) and CD132 (Uniprot: P31785-1). When stimulated with human IL-21 protein, the IL-21/ IL-21 R interaction drives STAT3-mediated luminescence. Inhibition of IL-21 binding to IL-21 R by either anti-IL-21 or anti-IL-21 R antibodies results in a decrease in luminescence.

• Screen for anti-human IL-21 or anti-human IL-21 R neutralizing antibody.

Adherent

DMEM medium + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.