HEK293/Human IDH1(132H)-P2A-mGFP&Luc Stable Cell Line

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The HEK293/Human IDH1(132H)-P2A-mGFP&Luc Stable Cell Line was engineered to not only express the full length human IDH1(Uniprot: O75874) mutated to a Histidine (H) at amino acid 132 and mGFP by a viral P2A self-cleaving peptide, but also express the firefly luciferase reporter. The expression of mGFP characterizing the human IDH1 (132H) was confirmed by flow cytometry. The Luciferase activity was confirmed by the detection of luminescence signal.

• Screen for human IDH1 inhibitor

• Used as target cells for luminescence-based cytotoxicity evaluation

Adherent

Puromycin (2 μg/mL) + Hygromycin (20 μg/mL)

DMEM + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.