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HEK293/Human GUCY2C Stable Cell Line

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产品详情

    1. Genetically modified cell lines best reflect MOA (Mechanism of Action)
    2. Higher activity and larger assay window for robust and reproducible cell-based bioassay
    3. Comprehensive application data to support assay development and validation
    4. Full tracible record, stringent quality control and validated cell passage stability
    5. Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
    6. Global commercial license assistance whenever regulatory filing is required

  • 描述（Description）

    The HEK293/Human GUCY2C Stable Cell Line was engineered to express the receptor full length human GUCY2C (Gene ID: 2984), used to mimic cancer target cells. Surface expression of human GUCY2C was confirmed by flow cytometry.

  • 应用说明（Application）

    • Useful for cell-based GUCY2C binding assay.

  • 生长特性（Growth Properties）

    Adherent

  • 筛选标记（Selection Marker）

    Puromycin (2 μg/mL)

  • 培养基（Complete Growth Medium）

    DMEM + 10% FBS

  • 冻存液（Freeze Medium）

    Serum-free cell cryopreservation medium

  • 装量（Quantity）

    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

  • 存储（Storage）

    Frozen in liquid nitrogen.

  • 支原体检测（Mycoplasma Testing）

    Negative

  • 无菌检测（Sterility Testing）

    Negative

  • 使用说明（Instructions for Use）

    See data sheet for detailed culturing and assay protocol.

产品展示

  • Receptor Assay

    GUCY2C FACS

    Expression analysis of human GUCY2C on HEK293/Human GUCY2C Stable Cell Line by FACS.
    Cell surface staining was performed on HEK293/Human GUCY2C Stable Cell Line or negative control cell using anti-human GUCY2C antibody followed by staining with PE anti-human IgG antibody.

    Protocol
*如有相关细胞池需求请联系我们

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背景介绍

GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP. Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. GUCY2C murine CAR-T cells recognized and killed human colorectal cancer cells endogenously expressing GUCY2C. Thus, we have identified a human GUCY2C-specific CAR-T cell therapy approach that may be developed for the treatment of GUCY2C-expressing metastatic colorectal cancer.

前沿进展

 
药物研发进展
  • 英文全称：

    Heat-stable enterotoxin receptor

  • 中文全称：

    热稳定肠毒素受体

  • 种类：

  • 上市药物数量：

    1 详情

  • 临床药物数量：

    5 详情

  • 最高研发阶段：

    批准上市

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