选择语言
免费注册
关注公众号
收藏产品

HEK293/Human GLP-1R Stable Cell Line (High Expression)

COAMS
DS
用户评价
货号-规格
价格
Qty.
CHEK-ATP160-2Vials (1Vial X 2)
询价
合计0件 产品金额¥ 0
新品建议
增项建议

产品详情

    1. Genetically modified cell lines best reflect MOA (Mechanism of Action)
    2. Higher activity and larger assay window for robust and reproducible cell-based bioassay
    3. Comprehensive application data to support assay development and validation
    4. Full tracible record, stringent quality control and validated cell passage stability
    5. Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
    6. Global commercial license assistance whenever regulatory filing is required

  • 描述（Description）

    The HEK293/Human GLP-1R Stable Cell Line was engineered to express the receptor full length human GLP-1R (Gene ID: 2740), with different levels of GLP-1R expression (High, Medium, Low). Surface expression of human GLP-1R was confirmed by flow cytometry.

  • 应用说明（Application）

    • Useful for cell-based GLP-1R binding assay

    • Screen for human GLP-1R agonists based on cAMP accumulation assay

  • 生长特性（Growth Properties）

    Adherent

  • 筛选标记（Selection Marker）

    Hygromycin B (20 μg/mL)

  • 培养基（Complete Growth Medium）

    DMEM + 10% FBS

  • 冻存液（Freeze Medium）

    Serum-free cell cryopreservation medium

  • 装量（Quantity）

    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

  • 存储（Storage）

    Frozen in liquid nitrogen.

  • 支原体检测（Mycoplasma Testing）

    Negative

  • 无菌检测（Sterility Testing）

    Negative

  • 使用说明（Instructions for Use）

    See data sheet for detailed culturing and assay protocol.

数据展示

  • Receptor Assay

    GLP1R FACS

    Expression analysis of human GLP-1R on HEK293/Human GLP-1R Stable Cell Line by FACS.
    Cell surface staining using PE-labeled anti-human GLP-1R antibody was performed on HEK293/Human GLP-1R Stable Cell Line with different expression levels: HEK293/Human GLP-1R Stable Cell Line (Low Expression); HEK293/Human GLP-1R Stable Cell Line (Medium Expression); HEK293/Human GLP-1R Stable Cell Line (High Expression).

    Protocol

  • Application

    GLP1R APPLICATION

    cAMP accumulation assay for human GLP-1R agonist screening.
    HEK293/Human GLP-1R Stable Cell Line (Low Expression), HEK293/Human GLP-1R Stable Cell Line (Medium Expression) and HEK293/Human GLP-1R Stable Cell Line (High Expression) were stimulated with Tirzepatide, respectively. The EC50 of Tirzepatide on HEK293/Human GLP-1R Stable Cell Line (High Expression) was approximately 0.0001544 μM.

    Protocol

  • Passage Stability

    GLP1R PASSAGE

    Passage stability analysis of receptors expression by FACS.
    Flow cytometry surface staining of human GLP-1R on HEK293/Human GLP-1R Stable Cell Line (High Expression) demonstrates consistent mean fluorescent intensity across passage 7-22.

    Protocol
*如有相关细胞池需求请联系我们

用户评价
发表评论

背景介绍

This gene encodes a 7-transmembrane protein that functions as a receptor for glucagon-like peptide 1 (GLP-1) hormone, which stimulates glucose-induced insulin secretion. This receptor, which functions at the cell surface, becomes internalized in response to GLP-1 and GLP-1 analogs, and it plays an important role in the signaling cascades leading to insulin secretion. It also displays neuroprotective effects in animal models. Polymorphisms in this gene are associated with diabetes. The protein is an important drug target for the treatment of type 2 diabetes and stroke.

前沿进展

 
药物研发进展
  • 英文全称：

    Glucagon-like peptide-1

  • 中文全称：

    胰高血糖素类肽-1

  • 种类：

  • 上市药物数量：

    0 详情

  • 临床药物数量：

    5 详情

  • 最高研发阶段：

    临床三期

联系我们
项目合作
查看更多项目
  • 产品基础信息
  • 产品详情
  • 数据展示
  • 用户评价
  • 背景介绍