Human GIPR (GFP) HEK293 Reporter Cell

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The Human GIPR (GFP) HEK293 Reporter Cell was engineered to not only express CREB signaling response element, but also express the receptor full length human GIPR (Uniprot: P48546-1), which can drive GFP expressing systems by GIPR agonists or glucose-dependent insulinotropic polypeptide (GIP) stimulation. In the absence of agonist or GIP, the GIPR receptor is not activated and fluorescence signal is low. In the presence of agonist or GIP, the GIPR pathway-activated fluorescence can be detected in a dose-dependent manner.

• Screen for agonists that can bind and activate GIPR

Adherent

Puromycin(2 μg/mL) + Hygromycin B(20 μg/mL)

DMEM + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.