Human GFRAL & RET (GDF15 receptor) (Luc) HEK293 Reporter Cell

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The Human GFRAL & RET (GDF15 receptor) (Luc) HEK293 Reporter Cell was engineered to not only express SRE signaling response element, but also express the receptors human GFRAL (Uniprot: Q6UXV0) and RET (Uniprot: P07949-1). When stimulated with human GDF15 protein, receptor-mediated signaling can drive SRE-mediated luminescence. Neutralization of biological effect of the ligand-receptor interaction by corresponding antibody results in a decrease in luminescence.

• Screen for neutralizing antibodies blocking the stimulation of human GDF15 protein

• Screen for anti-human GFRAL neutralizing antibodies

Adherent

Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL) + Zeocin (20 μg/mL)

DMEM + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.