HEK293/Human GCGR Stable Cell Line (Medium Expression)

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The HEK293/Human GCGR Stable Cell Line was engineered to express the receptor full length human GCGR (Uniprot: P47871), with different levels of GCGR expression (High, Medium, Low). Surface expression of human GCGR was confirmed by flow cytometry.

• Useful for cell-based GCGR binding assay

• Screen for human GCGR agonists based on cAMP accumulation assay

Adherent

Hygromycin B (20 μg/mL)

DMEM + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.