CHO/Human CD32b Stable Cell Line (Medium Expression)

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The CHO/Human CD32b Stable Cell Line was engineered to express the receptor full length human CD32b (Uniprot: P31994-1), with different levels of CD32b expression (High, Medium, Low), which can be used to test agonist antibody whether in a CD32b-dependent manner to strengthen the agonistic activity. When co-cultured with Human 4-1BB HEK293 Reporter Cell and anti-4-1BB agonist antibody, the anti-4-1BB antibody can be crosslinked, thereby strengthening 4-1BB pathway-activated luminescence.

• Useful for cell-based CD32b binding assay

• Useful for CD32b-mediated crosslinking

Adherent

Hygromycin B (20 μg/mL)

F-12K+ 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.