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HEK293/Human FAP Stable Cell Line

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产品详情

    1. Genetically modified cell lines best reflect MOA (Mechanism of Action)
    2. Higher activity and larger assay window for robust and reproducible cell-based bioassay
    3. Comprehensive application data to support assay development and validation
    4. Full tracible record, stringent quality control and validated cell passage stability
    5. Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
    6. Global commercial license assistance whenever regulatory filing is required

  • 描述（Description）

    The HEK293/Human FAP Stable Cell Line was engineered to express the full length human FAP (Uniprot: Q12884-1), used to mimic cancer target cells. Surface expression of human FAP was confirmed by flow cytometry.

  • 应用说明（Application）

    •        Useful for cell-based FAP binding assay

  • 生长特性（Growth Properties）

    Adherent

  • 筛选标记（Selection Marker）

    Puromycin (2 μg/mL)

  • 培养基（Complete Growth Medium）

    DMEM + 10% FBS

  • 冻存液（Freeze Medium）

    Serum-free cell cryopreservation medium

  • 装量（Quantity）

    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

  • 存储（Storage）

    Frozen in liquid nitrogen.

  • 支原体检测（Mycoplasma Testing）

    Negative

  • 无菌检测（Sterility Testing）

    Negative

  • 使用说明（Instructions for Use）

    See data sheet for detailed culturing and assay protocol.

产品展示

  • Receptor Assay

    FAP FACS

    Expression analysis of human FAP on HEK293/Human FAP Stable Cell Line by FACS.
    Cell surface staining was performed on HEK293/Human FAP Stable Cell Line or negative control cell using anti-human FAP antibody followed by staining with PE anti-human IgG Fc Antibody.

    Protocol

  • Passage Stability

    FAP PASSAGE

    Passage stability analysis of human FAP expression by FACS.
    Flow cytometry surface staining of human FAP on HEK293/Human FAP Stable Cell Line demonstrates consistent mean fluorescent intensity across passage 7-22.

    Protocol
*如有相关细胞池需求请联系我们

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背景介绍

FAP (also known as seprase) is a Type II transmembrane serine protease. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences. Degrade also gelatin, heat-denatured type I collagen. Also has dipeptidyl peptidase activity, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.

前沿进展

 
药物研发进展
  • 英文全称：

    Prolyl endopeptidase FAP

  • 中文全称：

    成纤维细胞激活蛋白α

  • 种类：

  • 上市药物数量：

    0 详情

  • 临床药物数量：

    39 详情

  • 最高研发阶段：

    临床二期

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