Human EGF R & (SRE-Luc) HEK293 Reporter Cell

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The Human EGF R & (SRE-Luc) HEK293 Reporter Cell expressing human EGF R was engineered to express signaling response element driving luciferase expressing systems. When stimulated with human EGF protein, the EGF/EGF R interaction drives SRE-mediated luminescence. Inhibition of biological effect of the ligand-receptor interaction by corresponding antibody or small molecule inhibitor results in a decrease in luminescence.

• Screen for anti-human EGF R or anti-human EGF neutralizing antibody.

• Screen for human EGF R small molecule inhibitor

Adherent

Puromycin (2 μg/mL)

DMEM medium + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.