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HEK293/Human EGF R Stable Cell Line

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产品详情

    1. Genetically modified cell lines best reflect MOA (Mechanism of Action)
    2. Higher activity and larger assay window for robust and reproducible cell-based bioassay
    3. Comprehensive application data to support assay development and validation
    4. Full tracible record, stringent quality control and validated cell passage stability
    5. Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
    6. Global commercial license assistance whenever regulatory filing is required
  • 描述(Description)

    The HEK293/Human EGF R Stable Cell Line was engineered to express the receptor full length human EGF R (Gene ID: 1956), used to mimic cancer target cells. Surface expression of human EGF R was confirmed by flow cytometry.

  • 应用说明(Application)

    • Useful for cell-based EGF R binding assay.

  • 生长特性(Growth Properties)

    Adherent

  • 筛选标记(Selection Marker)

    Puromycin (2 μg/mL)

  • 培养基(Complete Growth Medium)

    DMEM + 10% FBS

  • 冻存液(Freeze Medium)

    Serum-free cell cryopreservation medium

  • 装量(Quantity)

    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

  • 存储(Storage)

    Frozen in liquid nitrogen.

  • 支原体检测(Mycoplasma Testing)

    Negative

  • 无菌检测(Sterility Testing)

    Negative

  • 使用说明(Instructions for Use)

    See data sheet for detailed culturing and assay protocol.

产品展示

  • Receptor Assay

     EGF R FACS

    Expression analysis of human EGF R on HEK293/Human EGF R Stable Cell Line by FACS.
    Cell surface staining was performed on HEK293/Human EGF R Stable Cell Line or negative control cell using PE-labeled anti-human EGF R antibody.

    Protocol
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背景介绍

The epidermal growth factor receptor (EGFR) belongs to the ErbB family of receptor tyrosine kinases (RTK). The aberrant activity of EGFR has shown to play a key role in the development and growth of tumor cells, where it is involved in numerous cellular responses including proliferation and apoptosis.

前沿进展

 
药物研发进展
  • 英文全称:

    Epidermal growth factor receptor erbB1

  • 中文全称:

    表皮生长因子受体erbB1

  • 种类:

  • 上市药物数量:

    33 详情

  • 临床药物数量:

    182 详情

  • 最高研发阶段:

    批准上市

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