Human CD16a (158V) (Luc) Jurkat Reporter Cell (CD3 Negative)

1. Genetically modified cell lines best reflect MOA (Mechanism of Action)
2. Higher activity and larger assay window for robust and reproducible cell-based bioassay
3. Comprehensive application data to support assay development and validation
4. Full tracible record, stringent quality control and validated cell passage stability
5. Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
6. Global commercial license assistance whenever regulatory filing is required

Description

The Human CD16a (158V) (Luc) Jurkat Reporter Cell (CD3 Negative) was engineered to not only express the NFAT signaling response element and the receptor full length human CD16a (Uniprot: P08637) mutated to a Valine (V) at amino acid 158 exhibiting a higher affinity for IgG1 and IgG3 isotypes compared to CD16a-158F, but also targeted knockout of the endogenous TCR chains resulting in loss of functional TCR/CD3 complex on the cell surface. When co-cultured with a target cell and the corresponding anti-TAA × CD3 bispecific antibody (BsAb), the anti-TAA × CD3 BsAb simultaneously binds both the target-associated antigen (TAA) on the target cell and the CD16a (158V) receptor on the Human CD16a (158V) (Luc) Jurkat Reporter Cell (CD3 Negative), resulting in receptor clustering, intracellular signaling and NFAT-mediated luminescence.

Application

• Determination of ADCC activity induced by anti-TAA × CD3 BsAbs

Growth Properties

Suspension

Selection Marker

Puromycin (5 μg/mL) + Hygromycin (20 μg/mL) + Zeocin (20 μg/mL)

Complete Growth Medium

RPMI-1640 + 10% FBS

Freeze Medium

Serum-free cell cryopreservation medium

Quantity

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Storage

Frozen in liquid nitrogen.

Mycoplasma Testing

Negative

Sterility Testing

Negative

Instructions for Use

See data sheet for detailed culturing and assay protocol.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process