CHO/Human MS4A2 & FCER1G & FCER1A Stable Cell Line

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The CHO/Human MS4A2 & FCER1G & FCER1A Stable Cell Line was engineered to express the high-affinity IgE receptor, consisting of full length human MS4A2 (Uniprot: Q01362), FCER1G (Uniprot: P30273), and FCER1A (Uniprot: P12319). Surface expression of human FCER1A and its binding ability to human IgE were confirmed by flow cytometry.

• Useful for cell-based FCER1A binding assay

• Screen for neutralizing antibodies blocking the binding of human IgE

Adherent

Puromycin (2 μg/mL)

F-12K + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.