CHO/Human BTLA Stable Cell Line

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The CHO/Human BTLA Stable Cell Line was engineered to express the receptor full length human BTLA (Uniprot: Q7Z6A9-1), used to mimic cancer target cells. When co-cultured with human HVEM Reporter Cell, the BTLA/HVEM interaction drives NF-kB-mediated luminescence. Blocking the BTLA/HVEM interaction by either anti-BTLA or anti-HVEM antibodies results in a decrease in luminescence.

• Useful for cell-based BTLA binding assay

• Useful as BTLA-expressing target cells in reporter gene assay

Adherent

Puromycin (2 μg/mL)

F-12K + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.