Human APJ R (Luc) HEK293 Reporter Cell

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The Human APJ R (Luc) HEK293 Reporter Cell was engineered to not only express CREB signaling response element, but also express the receptor full length human Apelin receptor (APJ R) (Uniprot: P35414). In this reporter system, Forskolin stimulation induces the cAMP production, thereby driving CREB-mediated luminescence. In the presence of APJ R agonists or Apelin, which inhibits the forskolin-induced cAMP production, resulting in a decrease in luminescence.

• Screen for agonists that can bind and activate APJ R

Adherent

Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL)

DMEM + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.