Human AMY3R (CTR&RAMP3) (Luc) BHK 21 Reporter Cell Development Service

• Genetically modified cell lines best reflect MOA (Mechanism of Action)
• Higher activity and larger assay window for robust and reproducible cell-based bioassay
• Comprehensive application data to support assay development and validation
• Full tracible record, stringent quality control and validated cell passage stability
• Parental cell line legally obtained from internationally recognized cell resource bank and commercially licensed
• Global commercial license assistance whenever regulatory filing is required

The Human AMY3R (CTR&RAMP3) (Luc) BHK 21 Reporter Cell was engineered to not only express CREB signaling response element, but also express the human AMY3R generated by co-expressing the full length human calcitonin receptor (CTR) (Uniprot: P30988-2) and the full length human receptor activity-modifying protein 3 (RAMP3) (Uniprot: O60896) added with an mGFP tag, which can drive luciferase expressing systems by amylin receptor (AMYR) agonists or amylin stimulation. In the absence of agonist or amylin, the AMY3R receptor is not activated and luminescence signal is low. In the presence of agonist or amylin, the AMY3R pathway-activated luminescence can be detected in a dose-dependent manner.

• Screen for AMYR agonists that can bind and activate AMY3R.

Adherent

Puromycin (2 μg/mL) + Hygromycin B (20 μg/mL) + Zeocin (20 μg/mL)

DMEM + 10% FBS

Serum-free cell cryopreservation medium

1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium

Frozen in liquid nitrogen.

Negative

Negative

See data sheet for detailed culturing and assay protocol.