产品详情
抗体来源(Source)
Biotinylated Anti-SN38 Antibody, Mouse IgG1, Avitag is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
种属(Species)
Mouse
亚型(Isotype)
Mouse IgG1 & C-Avi | Mouse Kappa
偶联(Conjugate)
Biotin
抗体类型(Antibody Type)
Recombinant Monoclonal
免疫原(Immunogen)
SN38-OVA.
特异性(Specificity)
This product is a specific antibody specifically reacts with SN38.
应用(Application)
ApplicationRecommended UsageELISA0.1-125 ng/mL纯度(Purity)
90% as determined by SDS-PAGE.
90% as determined by SEC-MALS.
纯化(Purification)
Protein A purified / Protein G purified
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
产品展示
电泳(SDS-PAGE)
Biotinylated Anti-SN38 Antibody, Mouse IgG1, Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Anti-SN38 Antibody, Mouse IgG1, Avitag (Cat. No. SN8-BVM808) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Human TROP-2, His Tag (Cat. No. TR2-H5223) at 1 μg/mL, add Sacituzumab Govitecam in the 100% Human Serum and then add Biotinylated Anti-SN38 Antibody, Mouse IgG1, Avitag (Cat. No. SN8-BVM808) at 0.04 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
ProtocolImmobilized Sacituzumab Govitecam at 1 μg/mL (100 μL/well) can bind Biotinylated Anti-SN38 Antibody, Mouse IgG1, Avitag (Cat. No. SN8-BVM808) with a linear range of 0.1-8 ng/mL (Routinely tested).
Protocol
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