Monoclonal Anti-Human IgG Antibody FA-Labeled (for TR-FRET)

AIDD‑Ready
For detailed protocols, please download the TR-FRET Toolbox Experimental Guide.
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FRT-TA02-1000tests
$191.00
FRT-TA02-5000tests
$443.00
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FRT-TA02-20000tests
$1,391.00
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Application
The Monoclonal Anti-Human IgG Antibody FA-Labeled is a universal tool for TR-FRET assays that can bind human IgG or hFc-tagged proteins. This product uses specific anti-human IgG antibody labeled with fluorescent dyes and it can be used in combination with other Donors covalently conjugated with Eu³⁺ chelate. When the Donor and Acceptor come into close proximity (within a distance of less than 10 nm), a FRET reaction occurs: the 620 nm signal emitted by the Donor upon excitation by a specific light source is received by the Acceptor, which then emits a 665 nm signal.
Product Specifications
Source
This Monoclonal Anti-Human IgG Antibody is a mouse monoclonal antibody recombinantly expressed from Hybridoma.
Specificity
This antibody recognizes all four human IgG isotypes (IgG1, IgG2, IgG3, and IgG4) and multiple engineered variants, including: Human IgG1 (N297A), Human IgG1 (L234A, L235A), Human IgG1 (M252Y, S254T, T256E), Human IgG1 (L234F, L235E, P331S), Human IgG1 (S239D, I332E), Human IgG1 (S298A, E333A, K334A), Human IgG1 (T307A, E380A, N434A), Human IgG2 (A330S, P331S), Human IgG2 (P331S), Human IgG4 (S108P), and Human IgG4 (S228P, L235E).
Cross-Reactivity
Weak cross-reactivity with cynomolgus IgG, whereas no reactivity to human IgM, human IgA, mouse IgG isotypes (IgG1, IgG2a, IgG2b) or rabbit IgG.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Physical Appearance
Powder
Storage (unopened)
-20°C to -70°C
Storage (opened)
-70°C
Unit Size
1000 tests & 5000 tests & 20000 tests
Technology
TR-FRET
Intended Use
For binding the human IgG antibody or human Fc tagged protein as acceptor in TR-FRET Assay
Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.