APC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Site-specific conjugation)

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FM3-AY54P1-25tests
$450.00
In Stock - Delivery in 2-3 business days
FM3-AY54P1-200tests
$2,710.00
In Stock - Delivery in 2-3 business days
FM3-AY54P1
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APC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 is a neutralizing antibody produced via site-specific conjugation of APC to Monoclonal Anti-FMC63 Antibody, Mouse IgG1 under optimal conditions with a proprietary technology. We also carry another proclin-containing version of APC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (FM3-AY54A1) produced with the same production process except for proclin, and the proclin-containing product has the same performance and can be stored under 2-8 °C for no less than 12 months after reconstitution.

Clone

Y45

Isotype

Mouse IgG1, Kappa

Host Species

Mouse

Reactivity

Mouse

Immunogen

Recombinant FMC63 scFv derived from HEK293 cells

Conjugate

APC

Excitation Wavelength: 640 nm

Emission Wavelength: 661 nm

Specificity

Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.

Application

Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) CAR Expression). Please note that this product is NOT compatible to streptavidin detection system.

Recommended Dilution

1:50

Form

Lyophilized

Formulation

Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.5% BSA, with trehalose as protectant.

Please contact us for customized product forms or formulations.

Reconstitution

Please refer to the Certificate of Analysis (CoA) for specific instructions.

Shipping

Lyophilized product is shipped at ambient temperature.

Storage

For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 12 months under sterile conditions after reconstitution.
Notices

Product Specific Notices: For research use only.

バックグランド

FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.